P-156: Survey of In Vitro Effect of Resveratrol on Apoptosis of Human Endometrial Epithelial Cells

Background: In apoptosis is physiological cell death which eliminates injured and old cells. Any disruption in apoptosis, leads to disease. Resveratrol is a natural polyphenol with estrogen-like, antioxidant and antiinflammatory properties; also it showed cell prolifratory and inhibitory effect. The aim of present study was to determine resveratrol effect on apoptosis of human endometrial epithelial cells in in vitro culture. Materials and Methods: In this experiment study, human endometrial biopsies (n=8) were chopped in sterile conditions. Epithelial cells, were isolated after enzymatic digestion (collagenase 2 mg/ml), cell filtration though filter mesh. Cell viability was defined by trypan blue staining. The epithelial cells were divided into five groups, control, 1, 10, 20 and 50 μM resveratrol concentration for 48 hours in DMEM/F12 medium containing antibiotics. Cell apoptosis was determined with tunnel assay. Data was analyzed by one-way ANOVA and p<0.05 was considered significant. Results: Apoptosis were 5.9 ± 0.35, 8.4 ± 1.7, 10.03 ± 2.4, 15.46 ± 0.4 and 18.03 ± 1.9% in control, 1, 10, 20 and 50 μM resveratrol respectively and difference between groups was significant (p=0.015). Conclusion: Resveratrol can induce significant apoptosis in human endometrial epithelial cells in higher doses.